Harpreet Singh, Amrita Mishra and Arun K. Mishra Pages 213 - 218 ( 6 )
Background: In current era, plants are used worldwide to cure various ailments. There is an urgent need to search new plant derived bioactive agents that can be beneficial to cure helminthiasis. Therefore, we performed phytochemical screening, anthelmintic activity and anticonvulsant activity of Cleome viscosa Linn seeds.
Methods: Paralysis time (PT) and death time (DT) evaluation method was used to evaluate anthelmintic activity. For anticonvulsant activity, maximal electroshock induced seizures (MES) and pentylenetetrazol -induced (PTZ) models were used.
Results: Phytochemical screening revealed the presence of alkaloids, saponins, flavonoids, tannins and phenolic compounds in petroleum ether extract (PEE) and ethyl acetate extract (EAE). Cardiac glycosides, anthraquinone were present in PEE and terpenoids, steroids were present in EAE. At doses of 10 mg/ml, 25 mg/ml and 50 mg/ml, the extracts were evaluated against Pheretima posthuma (earthworm). The extracts showed dose-dependent anthelmintic effects on the earthworms. The findings suggested that no significant difference was observed between EAE (50mg/ml) and albendazole (reference drug, 10 mg/ml) against the earthworms. In view of anticonvulsant potential using PTZ model, PEE (400 mg/kg b.w.) and EAE (400 mg/kg b.w.) showed insignificant difference with hind limb tonic extension time as exhibited by standard treated animal (Phenytoin sodium 40 mg/kg b.w.). The findings of PTZ model suggested that latency to myclonic spasm and latency to clonic seizure as shown by EAE (400 mg/kg b.w.) was having no significant difference when comparision was made with Diazepam.
Conclusion: The outcome revealed that the EAE (400 mg/kg b.w.) is potent antihelminth and anticonvulsant.
Cleome viscosa Linn seeds, Pheretima posthuma, phytochemical screening, anthelmintic.
Central Facility of Instrumentation, School of Pharmaceutical Sciences, IFTM University, Moradabad, 244102