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Phytochemical Analysis, Antibacterial and Antioxidant Activity of Calotropis procera and Calotropis gigantea

[ Vol. 9 , Issue. 1 ]


Reena Rani, Dushyant Sharma, Monika Chaturvedi and Jaya Parkash Yadav*   Pages 47 - 60 ( 14 )


Background: Medicinal plants are having immense potential to cure various health ailments and used as drugs and remedies for the treatment of various diseases since civilization. Medicinal property of these plants lies in their secondary metabolites which covered various classes like phenols, alkaloids, flavonoids, tannins, etc. Besides this, these secondary metabolites serve as a prototype to synthesize the new synthetic drugs.

Objective: The present study was carried out to evaluate the antioxidant and antibacterial activity of leaves extracts of Calotropis procera and Calotropis gigantea and characterization of their bioactive metabolites by Fourier Transform Infrared (FTIR) spectroscopy and Gas Chromatography-Mass Spectroscopy (GC-MS).

Methods: Methanol, petroleum ether and water were used for the extract preparation using cold percolation method. Antibacterial activity was assessed by agar well diffusion assay. The antioxidant activity of both the plants of Calotropis species was carried out by using different assay. Phytochemical analysis was carried out by using FTIR spectroscopy and GC-MS analysis.

Results: Methanol extract of both the plants was found to possess highest antioxidant potential in comparison to other extracts. Methanol extract of C. gigantea and aqueous extract of C. procera showed the maximum antibacterial activity against the tested bacterial strains. FTIR analysis of plants extracts indicates the presence of phenolic compounds, alkanes, carboxylic acids, aldehydes, aliphatic and aromatic amines, allene, sulfoxides, phenyl ester nitrocompounds and imines. GC-MS analysis of C. procera aqueous extract showed the presence of R-limonene, mannosamine, tridecane, 1-bromo-, 2-propenoic acid, tridecyl ester, pentatriacontane and 1-hexacosene as major phytochemicals. C. gigantea methanol extract indicated the presence of hentriacontane, eicosane, 3,3- dimethylnonadecane, pentacosane, 1-hexacosene, pentatriacontane and clocortolone as major phytochemicals.

Conclusion: This study provides a systematic base for isolation of the novel bioactive phytochemicals from the Calotropis plant species and to evaluate their efficacy especially for antioxidant and antibacterial activities.


Antibacterial, antioxidant, Calotropis gigantea, Calotropis procera, DPPH assay, FTIR, GC-MS, total phenolic content.


Department of Genetics, M. D. University, Rohtak, 124001, Haryana, Department of Genetics, M. D. University, Rohtak, 124001, Haryana, Department of Genetics, M. D. University, Rohtak, 124001, Haryana, Department of Genetics, M. D. University, Rohtak, 124001, Haryana

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