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p53-Mediated Anticancer Activity of Citrullus colocynthis Extracts

Author(s):

Gaurav Joshi, Jatinder Kaur, Praveen Sharma, Gurpreet Kaur, Yuvraj Bhandari, Raj Kumar and Sandeep Singh*   Pages 1 - 9 ( 9 )

Abstract:


Current anticancer therapeutics come with significant side effects and thus the focus is shifting towards minimizing the side effects or to avoid the disease altogether. Thus, various natural products are being investigated for their potential therapeutic values which can be easily included in the daily diet of a person. Citrullus colocynthis (L.) fruit is commonly used in traditional medicines and is known to have antioxidant effects, thus it may possess potent anticancer activity as well. In the present study, it was found that seed and pulp extracts of the fruit are effective against various cancer cell lines while the normal cells, with lower rate of division, remain largely unaffected. The current study for the first time shows that these extracts function via regulation of p53 pathways and the mode of apoptosis is mostly via mitochondrial (intrinsic) pathway. The biological profiling of the extracts was also validated using molecular modelling studies utilizing the two major polyphenols constituents from colocynths i.e., Isoorientin and Isovitexin. The study suggested that the constituent has a multiple target approach for the inhibition of cancer cell proliferation and inhibition of ROS production via the major apoptotic proteins. All of these outcomes suggest and establish a critical role of ROS accumulation and mitochondrial function in the p53-dependent cell.

Keywords:

Citrullus colocynthis, Natural Products; Anticancer; Free Radicals, p53, Molecular Modelling

Affiliation:

Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Mansa Road, Bathinda 151001, Punjab, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Mansa Road, Bathinda 151001, Punjab, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Mansa Road, Bathinda 151001, Punjab, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Mansa Road, Bathinda 151001, Punjab, Maharishi Markandeshwar Institute of Medical Sciences and Research (MMIMSR), Ambala, Department of Pharmaceutical Sciences and Natural Products Central University of Punjab, Mansa Road, Bathinda 151001, Punjab, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Mansa Road, Bathinda 151001, Punjab



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