Babita Aggarwal*, Pankaj Sharma and H. S. Lamba Pages 1 - 6 ( 6 )
Cocos nucifera endocarp extracts were selected to characterize the bioactive components (phytochemicals) by the application of Gas chromatography and Mass spectrometric technique as well as to evaluate the antimalarial potential. Twenty compounds have been identified and characterized by GC-MS studies. Most of the compounds are polyphenolic in nature. Promising in vitro antiplasmodial activity was exhibited by both alcoholic (ACN) and hydroalcoholic (HACN) extracts against P. falciparum strains MRC-02 (CQ sensitive) with IC50 values < 5 μg/mL. To confirm the antimalarial potential, in vivo Peter’s 4-Day suppressive test using P. berghei strain was performed at a dose of 25 and 50 mg/kg/day for 4 consecutive days. HACN (% Suppression = 75.43 ± 0.18; MST=19.21 days) and ACN (% Suppression = 34.65 ± 0.11; MST=10.11 days) showed moderate activity (p < 0.05) at dose 50 mg/Kg, while standard drug chloroquine (8mg/kg) suppressed 100% parasitaemia. No morphological changes in erythrocytes were observed suggesting the non-toxicity of the extract. Gas chromatography–mass spectrophotometry analysis of HACN revealed phenolic compounds and flavonoids as chief bioactive compounds, exhibiting the antimalarial activity.
Cocos nucifera, Endocarp, P. falciparum, Antimalarial, GC MS analysis, Antiplasmodial
School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, School of Pharmaceutical Sciences, Jaipur National University, Jaipur, Rajasthan, Department of Pharmaceutical Chemistry, H. R. Institute of Pharmacy, Ghaziabad, Uttar Pradesh