Submit Manuscript  

Article Details


The Alkaloids and Bioactivities of Ethanol Extract from a Traditional Remedy of Vietnam

Author(s):

Nguyen Hoang Sa, Trinh Thi Thuy*, Tran Duc Quan, Dao Duc Thien, Nguyen Thanh Tam, Le Cong Hoan, Le Thi Hong Nhung, Huynh Thi Ngoc Ni, S. Adorisio and Domenico V. Delfino   Pages 1 - 6 ( 6 )

Abstract:


“Dai trang hoan ba Giang” (BG), which belong to herbalist “Ba Giang”, has been one of the famous herbal remedies for ulcerative colitis and diarrhea for over 100 years in Vietnam. However, until now, the main BG’s chemical components have not been investigated. In this study, dried powders of materials were extracted by 70% EtOH to afford an extract (DTBG). Two compounds were isolated from DTBG by using silica gel column chromatography. Their structures were identified as palmatine (1), berberine (2) by combining spectral analysis and comparison with reported data. Then, we carried out testing for DTBG on OCI cells, and DTBG was also subjected to a series of acute and subchronic toxicological tests to evaluate its safety. The results showed that DTBG inhibited the growth of OCI by a significant increase of caspase-8-independent apoptosis as measured by propidium iodide flow cytometry analysis and caspase-3 activation. In the acute study, DTBG is well tolerated, non-toxic and non-lethal to mice under the present experimental conditions. In the subchronic study in mice, DTBG did not impact on weight gain, blood, liver and kidney function.

Keywords:

Alkaloids, OCI cells, apoptosis, acute toxicity, subchronic toxicity.

Affiliation:

Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Institute of Chemistry, Vietnam Academy of Science and Technology, Institute of Chemistry, Vietnam Academy of Science and Technology, Graduate University of Science and Technology, Vietnam Academy of Science and Technology, University of Khanh Hoa, Hanoi University of Industry, Phu Yen University, Section of Pharmacology, Department of Medicine, University of Perugia, Perugia, Foligno Nursing School, Department of Medicine, University of Perugia, Perugia



Read Full-Text article