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Exploring the antimicrobial properties against human pathogens and mode of action of various extracts from Fredolia aretioides, an endemic medicinal plant of Morocco and Algeria

Author(s):

Btissam Bouchal, Mounia Elidrissi Errahhali, Manal Elidrissi Errahhali, Redouane Boulouiz, Meryem Ouarzane, Mariam Tajir, Katsuyoshi Matsunami* and Mohammed Bellaoui*  

Abstract:


Developing new antimicrobial medicines is one of the strategic objectives of the global action plan adopted by the World Health Assembly to tackle antimicrobial resistance. Considering the fact that natural products derived from medicinal plants are an important source for discovering new antimicrobial compounds, we investigated here the antimicrobial properties and the mode of action of various extracts from Fredolia aretioides. This plant is an endemic medicinal plant of Morocco and Algeria yet nothing is currently known about its antimicrobial properties against human pathogens. Various extracts from Fredolia aretioides were prepared and evaluated for their antibacterial activity against six bacterial species, and their antifungal activity against five fungi species. Chemical-genetic screens were performed using a collection of Saccharomyces cerevisiae viable haploid deletion mutants spanning diverse biological processes. The diethyl ether extract of roots was found to be active against Citobacter freundii, with a MIC of 400 μg/ml. Hydro-methanol, methanol and residual extracts from aerial parts and roots were active against all five fungi species tested. Our results showed that residual extracts were the most effective against the fungi tested. Residual extract from aerial parts was more potent than the residual root extract, with IC50’s of 60 µg/ml and 440 µg/ml, respectively. Chemical-genetic analysis in Saccharomyces cerevisiae revealed that residual extracts might affect fatty acid and sphingolipid biosynthesis. All these findings suggest that Fredolia aretioides is a promising source for the isolation of novel antimicrobial agents with novel mechanisms of action against human pathogens.

Keywords:

Fredolia aretioides, Antibacterial, Antifungal, Chemical-genetic, Sphingolipid

Affiliation:

Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Department of Pharmacognosy, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Genetics Unit, Faculty of Medicine and Pharmacy of Oujda, University Mohammed Premier, Oujda, Department of Pharmacognosy, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553



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