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Isolation of Cardamonin and Pinostrobin Chalcone from the Rhizomes of Boesenbergia rotunda (L.) Mansf. and their Cytotoxic Effects on H-29 and MDA-MB-231 Cancer Cell Lines

Author(s):

Ibrahim Awad Mohammed, Muhammad Nadeem Akhtar*, Foo Jhi Biau, Yin Sim Tor, Seema Zareen, Sakina Binti Shahabudin, Hazrulrizawati Binti Abd Hamid, Zaheer Ul Haq, Ruqaiya Khalil and Reem Maan Khalaf   Pages 1 - 8 ( 8 )

Abstract:


Background: Breast cancer and human colon cancer are the most common types of cancer in females and males, respectively. Breast cancer is the most common type of cancer after lung and colon cancers. Natural products are an important source for drug discovery. Boesenbergia rotunda (L.) Mansf. is commonly known as fingerroot, belongs to the Zingiberaceae family.

Objective: The aim of this study to isolate some natural compounds from the rhizomes of B. rotunda (L.) Mansf., and to investigate their cytotoxicity against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines.

Methods: The dried rhizomes of B. rotunda were extracted with methanol. The methanolic extract was further used for solvent-solvent extraction. Bioassay-guided extraction and isolation of the rhizomes of the B. rotunda exhibited the cytotoxic properties of hexane and dichloromethane fractions.

Results: Six major chemicals constituents, pinostrobin (1), pinostrobin chalcone (2), cardamonin (3), 4,5-dihydrokawain (4), pinocembrin (5), and alpinetin (6) were isolated from the rhizomes of the B. rotunda. All the chemical constituents were screened against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. The compound cardamonin (3) (IC50 = 5.62±0.61 and 4.44±0.66 μg/mL) and pinostrobin chalcone (2), (IC50 = 20.42±2.23 and 22.51±0.42 μg/mL) were found to be potent natural cytotoxic compounds against MDA-MB-231 and HT-29 colon cancer cell lines, respectively.

Conclusion: Cardamonin (3) and pinostrobin chalcone (2) were found to be the most potential natural compounds against breast cancer cell line MDA-MB-231 and colon cancer HT-29 cell line.

Keywords:

B. rotunda, Cytotoxic effects, H-29 and MDA-MB-231 Cancer cell lines, Molecular docking studies

Affiliation:

Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, 43400 Serdang, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Faculty of Medicine, Department of Pharmacology, University Kebangsaan Malaysia UKM Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras



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