Rishabha Malviya*, Pramod Kumar Sharma and Susheel Kumar Dubey Pages 1 - 21 ( 21 )
Objective: In the present study, an attempt was made to synthesize biodegradable, hemocompatible, antimicrobial and pH-responsive hydrogel.
Method: Microwave facilitated green synthesis was carried out for the grafting of acrylamide over Kheri gum polysaccharide (KGP) backbone. The grafted hydrogel was characterized in terms of FTIR spectra, 1H NMR spectra, SEM image, contact angle, chemical resistant, biodegradation, blood clotting time, thrombogenicity, hemolytic activity and cytotoxic effect.
Results: Graft copolymers (KGP-g-Am) showed more swelling in all the media like double distilled water, 1 N NaOH and 0.1 N HCl than native form. Graft copolymers showed more swelling in 1 N NaOH than 0.1 N HCl, followed by distilled water. Blood clotting studies showed longer clotting time for KGP-g-Am as compared to the uncoated glass surface and KGP coated glass surface. Results obtained after molecular docking predicts that TLR-4 receptors are significantly more responsible than TLR-2 receptors for antimicrobial activity of both KGP-g-Am and KGP. Experimental data clearly indicates the better antimicrobial efficacy of KGP-g-Am (K1) against Escherichia coli and Aspergillus niger than KGP. In molecular docking studies, KGP-g-Am showed prominent anticancer activity than KGP at the protease-activated receptor (PAR1). Results of In vitro cytotoxic activity against breast cancer cell lines (MCF 7), predicts better control over cell growth by KGP-g-Am (K1) as compared to KGP.
Conclusion: It can be concluded from the findings of the results that microwave assisted grafting over KGP backbone modulate and introduce prerequisite properties within the polymer and can be utilized for various biomedical, pharmaceutical and cosmaceutical applications.
polysaccharide, graft copolymer, pH-responsive, antimicrobial activity, kheri gum, cytotoxicity, receptor
Polymer Science Laboratory, Department of Pharmacy, School of Medical & Allied Sciences Galgotias University, Greater Noida U.P., Polymer Science Laboratory, Department of Pharmacy, School of Medical & Allied Sciences Galgotias University, Greater Noida U.P., Siddarth Institute of Pharmacy, Dehradun, Uttarakhand