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Antioxidant Activity and Chemical Composition of Helichrysum lacteum Coss. & Dur.

Author(s):

Souad Arrif*, Mohammed Benkhaled, Hamada Haba and Soumia Mouffouk   Pages 1 - 11 ( 11 )

Abstract:


Objective: The objective of the present study was to measure antioxidant activity and to evaluate phytochemical constitution of Helichrysum lacteum growing in Algeria.

Methods: The structure elucidation of the isolated compounds was performed by spectroscopic methods. Antioxidant activity of the extracts the crude extracts (PE, EtOAc and n-BuOH) and pure compound (noryangonin (3)) was investigated by 5 methods, including DPPH free radical scavenging activity, reducing power and ferric thiocyanate methods, hydrogen peroxide scavenging capacity and total antioxidant activity by phosphomolybdate assay.

Results: The chemical investigation of the EtOAc extract of the aerial parts of H. lacteum led to the isolation of two flavonoids, astragalin (1) and isoquercitrin (2), together with a styryl pyrone, noryangonin (3), and a cyclobutane dimer, achyrodimer C (4) as well as two triterpenoid compounds, β-sitosterol (5) and oleanolic acid (6). The highest phenolic and flavonoid contents were detected in the ethyl acetate extract. Noryangonin exhibited a highest antioxidant activity than those of the references and all the tested extracts, with a value of IC50 at 1.45±0.44 µg/mL in DPPH scavenging. In hydrogen peroxide scavenging essay, the results revealed that EtOAc extract exhibited the highest percent inhibition (63.15 %) as compared to the ascorbic acid (63.36 %) at the concentration of 100 µg/mL. In phosphomolybdenum method, the EtOAc extract showed the strongest activity (36.85±0.02 µg EAA/mg ex) as compared to those of n-BuOH and PE extracts.

Conclusion: The antioxidant property shown in these findings needs further investigation, especially with the pure compound (3).

Keywords:

Helichrysum lacteum, flavonoid, NMR, antioxidant activity.

Affiliation:

Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Science of matter, Batna-1 University, Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Science of matter, Batna-1 University, Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Science of matter, Batna-1 University, Laboratory of Chemistry and Environmental Chemistry (L.C.C.E), Department of Chemistry, Faculty of Science of matter, Batna-1 University



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