Eman F Ahmed*, Amany A Sleem, Fawkeya A Abbas, Azza M El-Shafae and Maher M El-Domiaty Pages 1 - 18 ( 18 )
Background: Prosopis farcta (Banks & Sol.) J. F. Macbr. is a prickly perennial shrub belonging to family Leguminosae. Literature survey revealed that there are no sufficient phytochemical and biological studies on the roots and rhizomes of this plant, therefore, the present work aimed at their phytochemical and biological evaluation.
Methods: The shade-dried roots and rhizomes were powdered together, extracted by 85% ethanol and subjected to phytochemical investigation. Biologically, the antioxidant, antidiabetic, cytotoxic, antiallergic and antimicrobial activities were evaluated.
Results: The phytochemical investigation resulted in the isolation of 14 compounds including the fatty acid derivative, threo- methyl 9, 10-dihydroxyoctadecanoate (5), that is isolated for the first time from a natural source, and the identification of 47 compounds by HPLC-PDA-ESI-MS/MS analysis including organic acids and their glycosides, flavonoids, anthraquinones and lignan derivatives. Biologically, threo- methyl 9, 10-dihydroxyoctadecanoate (5) exerted a potent cytotoxic effect against human lung carcinoma (A-549) and human colon carcinoma (HCT-116) cell lines. The total alcoholic extract showed a strong DPPH scavenging activity, a significant decrease in the blood glucose level in alloxan-induced diabetic rats and a mild antibacterial effect against Bacillus subtillis, Staphylococcus aureus (G +ve bacteria) and Escherichia coli (G –ve bacteria).
Conclusion: This is the first report on the isolation and identification of threo- methyl 9, 10-dihydroxyoctadecanoate (5) from a natural source, and this novel compound exhibited potent cytotoxic activities against A-549 and HCT-116 cell lines. Moreover, this is the first HPLC-PDA-ESI-MS/MS profiling for this plant.
Prosopis farcta, HPLC-PDA-ESI-MS/MS analysis, antioxidant, antidiabetic, cytotoxic
Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Pharmacology Department, National Research Centre, El-Behoose St., El-Dokki, Giza, Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig, Pharmacognosy Department, Faculty of Pharmacy, Zagazig University, 44519 Zagazig