Ranjitsinh Devkar*, Jaymesh Thadani, Shridhar Sanghvi, Kiran Lagu and Kavita Shirsath Pages 333 - 339 ( 7 )
Aim: The present study aims to compare the cytoprotective effect of Cuminum cyminum L. (CC) extract and cuminaldehyde (CA) against lipotoxicity induced by oxidized low density lipoprotein (Ox-LDL) in mouse macrophage (RAW 264.7) cells.
i) To assess comparative Cytoprotective potential of CC and CA against Ox-LDL induced cytotoxicity.
ii) To study efficacy of CC and CA in preventing Ox-LDL induced apoptosis.
Methods: Protective effect of CC extract and CA aganist Ox-LDL induced cytotoxicity in RAW 264.7 cells was assessed by MTT assay. DCFDA stain was used to check the generation of ROS followed by analysis of apoptotic genes by quantitative RT-PCR.
Results: CC extract was found to be non-toxic up to 300 μg/ml but CA showed significant toxicity from 50 to 300 μg/ml. Cells treated with Ox-LDL recorded 80 % decrement in cell viability as compared to the control cells. But Ox-LDL+CC treated group accounted for improved cell viability (88 %) which was comparable to that of control. However, Ox-LDL+CA treated cells did not record any improvement in cell viability (19 %). DCF-DA staining revealed that the presence of CC could minimize intracellular oxidative stress but similarly this was persistent in CA supplemented group. Furthermore, mRNA expression of apoptotic genes revealed that Ox-LDL induced upregulation of Bax and downregulation of Bcl-2 genes were not recorded in Ox-LDL+CC treated group.
Conclusion: It can be concluded that CC extract efficiently prevented Ox-LDL induced lipotoxicity and apoptosis and has an anti-atherosclerotic potential. The failure of CA emphasizes the importance of naturally occurring polyherbal formulations over pure compounds in imparting bioactivity and for therapeutic applications.
Cuminum cyminum L., Cuminaldehyde, LDL oxidation, macrophage, apoptosis, oxidative stress.
Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat, Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat, Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, Formulation Development, Alvogen, 10B, Bloomfield Avenue, Pine Brook, NJ, Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat