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Cuminum cyminum Prevents Lipotoxicity and Apoptosis but Cuminaldehyde Fails to Do So: A Study on Mouse Macrophage (RAW 264.7) Cells

Author(s):

Ranjitsinh Devkar*, Jaymesh Thadani, Shridhar Sanghvi, Kiran Lagu and Kavita Shirsath   Pages 1 - 7 ( 7 )

Abstract:


The present study compares cytoprotective effect of Cuminum cyminum L. (CC) extract and cuminaldehyde (CA) against lipotoxicity induced by oxidized low density lipoprotein (Ox-LDL) in mouse macrophage (RAW 264.7) cells. CC extract was found to be non-toxic up to 300 µg/ml but CA showed significant toxicity from 50 to 300 µg/ml. Cells treated with Ox-LDL recorded 80 % decrement in cell viability as compared to the control cells. But Ox-LDL+CC treated group accounted for improved cell viability (88 %) which was comparable to that of control. However, Ox-LDL+CA treated cells did not record any improvement in cell viability (19 %). DCF-DA staining revealed that presence of CC could minimize intracellular oxidative stress but the same was persistent in CA supplemented group. Further, mRNA expression of apoptotic genes revealed that Ox-LDL induced upregulation of Bax and downregulation of Bcl-2 genes but such changes were not recorded in Ox-LDL+CC treated group. It can be surmised that CC extract efficiently prevented Ox-LDL induced lipotoxicity and apoptosis and has anti-atherosclerotic potential. Failure of CA emphasizes the importance of naturally occurring polyherbal formulations over pure compounds in imparting bioactivity and for therapeutic applications.

Keywords:

Cuminum cyminum L., Cuminaldehyde, LDL oxidation, Macrophage, Apoptosis, Oxidative stress

Affiliation:

Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, GujaratDivision of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat, Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat, Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, PA 19102, Formulation Development, Alvogen, 10B, Bloomfield Avenue, Pine Brook, NJ, Division of Phytotherapeutics and Endocrinology, Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara – 390002, Gujarat



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