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The Effects of Hydroalcoholic Extract from Alhagi on Matrix Metalloproteinase- 9 Production in Peripheral Blood Mononuclear Cells from Patients with Rheumatoid Arthritis

Author(s):

Mahbouba Ahmadi, Reyhane Ebrahimi, Mohammad Ansari, Masoumeh Akhlaghi, Mahdi Mahmoudi, Gholamreza Amin and Solaleh Emamgholipour*   Pages 1 - 6 ( 6 )

Abstract:


Background: Up-regulation of matrix metalloproteinase 9 (MMP-9) along with imbalance in ratio of MMP-9 relative to tissue inhibitor of metalloproteinase 1 (TIMP-1) is important in the pathogenesis of rheumatoid arthritis (RA). Here, we investigated whether hydroalcoholic extract from root of Alhagi camelorum Fisch can affect the levels of MMP-9 and TIMP-1 in peripheral blood mononuclear cells (PBMCs) of RA patients.

Objective: In the current study, we suggest that Alhagi may have an inhibitory effect on MMP-9 production, which is mainly responsible for joint destruction in RA. In addition, we would like to stress that our findings, along with others, can provide the basis for future studies, which might be able to declare the role of chemical ingredients of Alhagi as therapeutic targets for RA treatment.

Method: PBMCs were isolated from 12 RA patients and 12 healthy subjects and treated with two concentrations of Alhagi extract (100 and 500 µg/ml) for 24 h. MMP-9 expression and activity, TIMP-1 levels and nitric oxide (NO) production were evaluated using standard methods.

Results: Alhagi (500 µg/ml) caused a significant reduction in expression and activity of MMP-9 in PBMCs from healthy (p=0.05 and p=0.003 respectively) and patient (p= 0.003 and p=0.02 respectively) subjects. Moreover, Alhagi (100 µg/ml) decreased MMP-9 production in healthy group (p=0.02).

Conclusion: It appears that Alhagi could be beneficial in the control of inflammation and joint destruction in RA at least partly by inhibition of MMP-9 production.

Keywords:

Matrix metalloproteinase 9 (MMP-9), Tissue inhibitor of metalloproteinase 1 (TIMP-1), Rheumatoid arthritis (RA), Alhagi camelorum Fisch, Peripheral blood mononuclear cells (PBMCs)

Affiliation:

Biochemistry Department, Tehran University of Medical Sciences, Tehran, Biochemistry Department, Tehran University of Medical Sciences, Tehran, Biochemistry Department, Tehran University of Medical Sciences, Tehran, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Pharmacognosy and Medicinal Plant Research Center Department, Tehran University of Medical Sciences, Tehran, Biochemistry Department, Tehran University of Medical Sciences, Tehran



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