Areeg M. Abdelrazek* and Shimaa A. Haredy Pages 1 - 9 ( 9 )
Background: Busulfan (Bu) is an anticancer drug-induced a variety of adverse effects for cancer patients. Oxidative stress has been considered as a common pathological mechanism and it has a key role in the initiation and progression of liver injury by Bu.
The Aim: To evaluate the antioxidant Impact of L-carnitine and Coenzyme Q10 and its protective role against oxidative stress damage occur in liver tissues.
Methods and Material: Thirty-six albino rats were divided equally into six groups. G1 (con), received I.P. injection of DMSO plus 1 ml of distilled water daily by oral gavages; G2 (Bu), received I.P. injection of Bu plus 1 ml of the distilled water daily; G3 (L-car), received 1 ml of L-car orally; G4 (Bu + L-car) received I.P. injection of Bu plus 1 ml of L-car, G5 (COQ10) 1 ml of CoQ10 daily; G6 (Bu + COQ10) received I.P. injection of Bu plus 1 ml of CoQ10 daily.
Results: The recent data showed that Bu-induced significant (P<0.05) elevation in serum ALT, AST, liver GSSG, NO, MDA and 8-OHdG, while showed significant (P<0.05) decrease in liver GSH and ATP.
On the other hand, L-carnitine and Coenzyme Q10 ameliorated the negative effects prompted by Bu. Immunohistochemical expression of caspase-3 in liver tissues reported pathological alterations in Bu group while showed significant recovery in L-car more than CoQ10.
Busulfan, CoQ10, L-carnitine, Oxidative stress, Caspase-3, Hepatotoxicity
Researcher at Physiology Department at National Organization of Drug Control and Research, Researcher at Physiology Department at National Organization of Drug Control and Research