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Amine Derivative from the Aerial Part of Spilanthes acmella Murr. and their Alkaline Phosphatase Activity

Author(s):

Retno Widyowati*, Wiwied Ekasari and Neny Purwitasari   Pages 1 - 7 ( 7 )

Abstract:


Background: Spilanthes acmella Murr. is included in Asteraceae, used as a traditional remedy for tooth-aches and originates from Africa, America, Borneo, India, Sri Lanka, Bangladesh China, Japan, Thailand and Indonesia

Methods: The air-dried plant of Spilanthes acmella Murr. was extracted with methanol, then partitioned with n-hexane and ethyl acetate, successively by using liquid-liquid extraction and repeated chromatographic techniques, such as silica gel, octadecyl silylated silica gel (ODS) and HPLC. The isolated compounds were determined by spectrometric analysis using UV, infrared (IR), high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D, and 2D NMR.

Results: A benzenepropanoic acid, 4 hydroxy-2-oxo-3 piperidinyl ester (1) was isolated from the ethyl acetate layer of whole plant of Spilanthes acmella Murr. together with dendranthemoside A (2), uridine (3), icariside B2 (4), chicoriin (5), dendranthemoside B (6), ampelopsisionoside (7) and benzyl-β-L-arabinopyranosyl (1→6)-β-D-glucopyranoside (8) from their butanol layer.

Conclusion: The ethyl acetate layer of Spilanthes acmella contains an amine derivative, a benzenepropanoic acid, 4 hydroxy-2-oxo-3 piperidinyl ester (1), it was reported for the first time from this plant and naturally.

Keywords:

Spilanthes acmella Murr., Asteraceae, benzenepropanoic, ethyl acetate, structure elucidation, ALP activity

Affiliation:

Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, AirlanggaUniversity, Dharmawangsadalam, Surabaya, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, AirlanggaUniversity, Dharmawangsadalam, Surabaya, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, AirlanggaUniversity, Dharmawangsadalam, Surabaya



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