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Characterization of Galactomannan Isolated from Algerian Gleditsia triacanthos L. Seeds


Mourad Leboukh*, Saoudi Aouadi* and Hicham Gouzi   Pages 1 - 7 ( 7 )


Background: The galactomannans are a water-soluble carbohydrates widely used in food, pharmaceutical, biomedical, cosmetic, textile and paper industries. Recently, a need for alternative industrial source of galactomannans arises such as Honey locust (Gleditsia triacanthos) which is abundant in Algeria.

Objective: The main objective of this study was to determinate physico-chemical and rheological properties of water-soluble galactomannan (WSG) purified from seeds of Algerian honey locust (Gleditsia triacanthos L.).

Methods: The galactomannan was purified from the seed of Gleditsia triacanthos and structurally characterized by chromatographic (FT-IR, 1H, 13C NMR) and spectroscopic (HPAEC, HPSEC-RID-MALLS) methods.

Results: The Honey locust seed contains a high level of WSG (yield, 10%) and its molecular weight for WSG was approximately 871.9 kDa. A highly viscous solution was obtained which had a intrinsic viscosity [η] of 7.14-7.25 dL/g and optical activity [α]D of +38.7°. Chromatographic and spectroscopic analysis indicated the presence of β-D-mannopyranose and α-D-galactopyranose in the heteropolysaccharide at a molar ratio of 2.86 : 1. The linear backbone of its macromolecule has a central core composed of 4-linked β-mannose units, with branches of galactose, linked to the carbohydrate core through α(1–6) linkage.

Conclusion: These results lead to the conclusion that galactomannan presents a good physico-chemical characteristics suitable for applications in pharmaceutical, cosmetic and food industries.


Gleditsia triacanthos, Galactomannan, extraction, purification, characterization


Ecole Normale Superieure (ENS) de Ouargla, 30000, Laboratoire de Biochimie et Microbiologie Appliquée (LBMA), Département de Biochimie, Faculté des Sciences, Université Badji Mokhtar, BP12, 23000 Annaba, Université Amar Télidji, Faulté des Sciences, Département de Biologie, Route de Ghardaia BP G37, Laghouat 03000

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