Nabila Souilah, Hamdi Bendif, Zain Ullah, Mohamed Djamel Miara, Messaoud Laib, Mehmet Öztürk, Salah Akkal, Kamel Medjroubi and Ahmed M. Mustafa*
Introduction: Oenanthe fistulosa L. (Apiaceae) is often associated with damp soils. Its underground parts and the young leaves are mainly cooked with other vegetables.
Objective: The aim of the current work was to investigate the chemical profile of dichloromethane (DCM), ethyl acetate (EA) and n-butanol (BuOH) fractions of O. fistulosa through analysis of 37 phytochemicals by LC-MS/MS and to evaluate their biological activities such as antioxidant, anticholinesterase and antityrosinase for the first time.
Methods: Analysis of 37 phytochemicals was performed by liquid chromatography-mass spectrometry (LC-MS/MS). Antioxidant activity was evaluated using five in vitro assays, while anti-cholinesterase and anti-tyrosinase activities were performed using Ellman and Dopachrome methods, respectively.
Results: The number of phenolic compounds detected in DCM, EA and BuOH fractions were found to be 9, 15, and 12; respectively. More specifically, 9 phenolic acids were detected and among them chlorogenic, tr-ferulic and p-coumaric acids were the most abundant. While 8 flavonoids were detected and apigetrin, rutin, and quercitrin were the most abundant. In addition, 3 non-phenolic organic acids (quinic, malic and fumaric acids) were detected in large quantities. Furthermore, the tested plant fractions demonstrated a noteworthy and strong antioxidant action. The plant displayed very strong action against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes; and BuOH fraction was the most potent one. Finally, BuOH and DCM fractions showed good tyrosinase inhibitory activity.
Conclusion: According to the obtained results, O. fistulosa might be a promising candidate for aleviation of oxidative stress, neurodegenerative (such as Alzheimer’s disease) and hyperpigmentation disorders.
Oenanthe fistulosa, Apiaceae, Polyhenolics, LC-MS/MS, Antioxidant, Anticholinesterase, Antityrosinase
Department of Chemistry, Faculty of Exact Sciences, University of Constantine 1, Constantine, Department of Natural and Life Sciences, Faculty of Sciences, University of M’sila, BP 166 M’sila, 28000, Department of Chemistry, Mugla Sitki Koçman University, Kötekli-48000, Department of Biology, University of Tiaret 14000. DZ, Tiaret, Department of Natural and life Sciences, Faculty of Sciences, University of Skikda, Skikda, Department of Chemistry, Muğla Sitki Koçman University, Kötekli-48000, Muğla, Department of Chemistry, Faculty of Exact Sciences, University of Constantine 1, Constantine, Department of Chemistry, Faculty of Exact Sciences, University of Constantine 1, Constantine, Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig–44519