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Punica granatum L. inhibits the growth of microorganisms associated with bovine mastitis

Author(s):

Tauana F. Freitas, Fernanda M. Marques, Ricardo M. Kuster, João B. Salles, Cristiane P. Victório* and Maria Cristina de Assis  

Abstract:


Background: The current research aimed to explore secondary metabolites and antibacterial activity against bacterial agents associated with bovine mastitis from extracts prepared from the peel of fruits of Punica granatum L.. Mastitis is an inflammation in the mammary gland mainly due to infection of bacteria and can be presented clinically or subclinically. The infectious bovine mastitis disease is associated with the reduction and changes in the composition of the milk, as well as in the early disposal of the animal.

Objective: We describe the bactericidal activity of ethanolic extracts of the fruit peel of P. granatum, against etiological agents of bovine mastitis (Escherichia coli, Staphylococcus aureus and Streptococcus agalactiae).

Method: The bactericidal activity was evaluated according to the standards of the European Committee for Antimicrobial Susceptibility Testing and by the broth microdilution methodology according to the standards of the Clinical and Laboratory Standards Institute. The fruit peel extracts of P. granatum were prepared with ethanol solvent (90%).

Results: The best results of total inhibition of bacterial growth of the three strains tested were obtained with the concentrations of 75mg/mL of the extract after 1 and 3 hours of treatment. Cell membrane integrity analysis by flow cytometry showed that 1 h of contact there was loss of integrity of the wall and cell membranes.

Conclusion: We can suggest that the ethanolic extract of the fruit peel of P. granatum can be an important natural coadjutant compound in the treatment of mastitis as well as being used in the formulation of sanitizers.

Keywords:

Antibacterial Activity, Antibiotic resistance, Punicalagin, Pathogenic Organism, Staphylococcus, Plant Products

Affiliation:

Laboratory of Research in Environmental Biotechnology, Fundação Centro Universitário Estadual da Zona Oeste [UEZO], Campo Grande, RJ, Pharmaceutical Didactic Laboratory - Pharmaceutical Sector and Quality Control, Fundação Centro Universitário Estadual da Zona Oeste [UEZO], Campo Grande, RJ, Departament of Chemistry – Universidade Federal do Espírito Santo [UFES],Vitória, ES, Laboratory of Biochemistry, Fundação Centro Universitário Estadual da Zona Oeste, Rio de Janeiro, Laboratory of Research in Environmental Biotechnology, Fundação Centro Universitário Estadual da Zona Oeste [UEZO], Campo Grande, RJ, Laboratory of Research in Environmental Biotechnology, Fundação Centro Universitário Estadual da Zona Oeste [UEZO], Campo Grande, RJ



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